Quantitative characterization of single-cell adhesion properties by atomic force microscopy using protein-functionalized microbeads.
Fiche publication
Date publication
novembre 2018
Journal
Journal of molecular recognition : JMR
Auteurs
Membres identifiés du Cancéropôle Est :
Pr DEDIEU Stéphane, Dr DEVY Jérôme, Dr BERQUAND Alexandre
Tous les auteurs :
Chièze L, Le Cigne A, Meunier M, Berquand A, Dedieu S, Devy J, Molinari M
Lien Pubmed
Résumé
A method was developed to characterize the adhesion properties of single cells by using protein-functionalized atomic force microscopy (AFM) probes. The quantification by force spectroscopy of the mean detachment force between cells and a gelatin-functionalized colloidal tip reveals differences in cell adhesion properties that are not within reach of a traditional bulk technique, the washing assay. In this latter method, experiments yield semiquantitative and average adhesion properties of a large population of cells. They are also limited to stringent conditions and cannot highlight disparities in adhesion in the subset of adherent cells. In contrast, this AFM-based method allows for a reproducible and quantitative investigation of the adhesive properties of individual cells in common cell culture conditions and allows for the detection of adhesive subpopulations of cells. These characteristics meet the critical requirements of many fields, such as the study of cancer cell migratory abilities.
Mots clés
atomic force microscopy (AFM), cancer, cell adhesion, cell migration, force spectroscopy, tip functionalization
Référence
J. Mol. Recognit.. 2018 Nov 7;:e2767