Elastin receptor (S-gal) occupancy by elastin peptides modulates T-cell response during murine emphysema.
Fiche publication
Date publication
septembre 2017
Journal
American journal of physiology. Lung cellular and molecular physiology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr LE NAOUR Richard
Tous les auteurs :
Meghraoui-Kheddar A, Pierre A, Sellami M, Audonnet S, Lemaire F, Le Naour R
Lien Pubmed
Résumé
Chronic obstructive pulmonary disease and emphysema are associated with increased elastin peptides (EP) production because of excessive breakdown of lung connective tissue. We recently reported that exposure of mice to EP elicited hallmark features of emphysema. EP effects are largely mediated through a receptor complex that includes the elastin-binding protein spliced-galactosidase (S-gal). In previous studies, we established a correlation between cytokine production and S-gal protein expression in EP-treated immune cells. In this study, we investigated the S-gal-dependent EP effects on T-helper (Th) and T-cytotoxic (Tc) responses during murine EP-triggered pulmonary inflammation. C57BL/6J mice were endotracheally instilled with the valine-glycine-valine-alanine-proline-glycine (VGVAPG) elastin peptide, and, 21 days after treatment, local and systemic T-lymphocyte phenotypes were analyzed at cytokine and transcription factor expression levels by multicolor flow cytometry. Exposure of mice to the VGVAPG peptide resulted in a significant increase in the proportion of the CD4 and CD8 T cells expressing the cytokines IFN-γ or IL-17a and the transcription factors T-box expressed in T cells or retinoic acid-related orphan receptor-γt (RORγt) without effects on IL-4 and Gata-binding protein 3 to DNA sequence [A/T]GATA[A/G] expression. These effects were maximized when each T-cell subpopulation was challenged ex vivo with EP, and they were inhibited in vivo when an analogous peptide antagonizing the EP/S-gal interactions was instilled together with the VGVAPG peptide. This study demonstrates that, during murine emphysema, EP-S-gal interactions contribute to a Th-1 and Th-17 proinflammatory T-cell response combined with a Tc-1 response. Our study also highlights the S-gal receptor as a putative pharmacological target to modulate such an immune response.
Mots clés
Amino Acid Sequence, Animals, Bronchoalveolar Lavage Fluid, CD8-Positive T-Lymphocytes, immunology, Elastin, chemistry, Female, Galactosidases, antagonists & inhibitors, Interferon-gamma, metabolism, Interleukin-17, metabolism, Lymph Nodes, pathology, Lymphocyte Count, Mice, Inbred C57BL, Models, Biological, Pancreatic Elastase, metabolism, Peptides, chemistry, Pulmonary Emphysema, immunology, Spleen, pathology, Sus scrofa, T-Lymphocytes, immunology, T-Lymphocytes, Cytotoxic, drug effects, Th1 Cells, immunology, Th17 Cells, immunology
Référence
Am. J. Physiol. Lung Cell Mol. Physiol.. 2017 Sep 1;313(3):L534-L547