Dual 0.02% chlorhexidine digluconate - 0.1% disodium EDTA loaded thermosensitive ocular gel for Acanthamoeba keratitis treatment.
Fiche publication
Date publication
décembre 2018
Journal
International journal of pharmaceutics
Auteurs
Membres identifiés du Cancéropôle Est :
Mr RISLER Arnaud
Tous les auteurs :
Cucina A, Filali S, Risler A, Febvay C, Salmon D, Pivot C, Pelandakis M, Pirot F
Lien Pubmed
Résumé
Poor bioavailability and low residence time limit the efficiency of conventional biguanide-based eye drops against Acanthamoeba keratitis. The aim of this work was to formulate an original anti-amoebic thermoreversible ocular gel combining biguanide and metalloproteases inhibitor - chelating agent. Chlorhexidine digluconate (CHX)-ethylenediaminetetraacetic acid disodium salt (NaEDTA) were compounded in poloxamer 407 saline solution. 0.02% CHX - 0.1% NaEDTA loaded thermosensitive ocular gel exhibited appropriate pH (5.73 ± 0.06), iso-osmolality (314 ± 5 mOsm/kg), viscosity (ranged between 15-25 mPa.s) and thermal gelation (26.5°C and 33°C) properties. Bioadhesion of gel was successfully tested onto isolated bovine eyes as well as the assessment of CHX penetration into the cornea. Intracorneal CHX concentration was found greater than trophozoite minimum amoebicidal concentration and minimal cysticidal concentration after 15-min and 2-h ocular exposure, respectively, while any CHX permeation through the cornea was detected (< 51 ng/cm/h). Improvement of CHX ocular bioavailability was attributed to probable solubilization of tear film lipid layer by poloxamer. In vitro efficiency of CHX-NaEDTA ocular gel was confirmed from the drastic reduction of trophozoite and cyst survival (to 25% and 2%, respectively), confirming the potential of the multicomponent pharmaceutical material strategy for the treatment of Acanthamoeba keratitis.
Mots clés
Acanthamoeba, Chlorhexidine, EDTA disodium salt, Eye drops, Keratitis, Poloxamer
Référence
Int J Pharm. 2018 Dec 12;: