Comparative Characterization of Phosphatidic Acid Sensors and Their Localization during Frustrated Phagocytosis.
Fiche publication
Date publication
mars 2017
Journal
The Journal of biological chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France, Dr CHASSEROT-GOLAZ Sylvette, Dr HEINTZ Dimitri, Dr VITALE Nicolas
Tous les auteurs :
Kassas N, Tanguy E, Thahouly T, Fouillen L, Heintz D, Chasserot-Golaz S, Bader MF, Grant NJ, Vitale N
Lien Pubmed
Résumé
Phosphatidic acid (PA) is the simplest phospholipid naturally existing in living organisms, but it constitutes only a minor fraction of total cell lipids. PA has attracted considerable attention because it is a phospholipid precursor, a lipid second messenger, and a modulator of membrane shape, and it has thus been proposed to play key cellular functions. The dynamics of PA in cells and in subcellular compartments, however, remains an open question. The recent generation of fluorescent probes for PA, by fusing GFP to PA-binding domains, has provided direct evidence for PA dynamics in different intracellular compartments. Here, three PA sensors were characterized in vitro, and their preferences for different PA species in particular lipidic environments were compared. In addition, the localization of PA in macrophages during frustrated phagocytosis was examined using these PA sensors and was combined with a lipidomic analysis of PA in intracellular compartments. The results indicate that the PA sensors display some preferences for specific PA species, depending on the lipid environment, and the localization study in macrophages revealed the complexity of intracellular PA dynamics.
Mots clés
Phospholipase D, biosensor, fatty acid, lipid signaling, phagocytosis, phosphatidic acid
Référence
J. Biol. Chem.. 2017 Mar;292(10):4266-4279