Phospholipase D1: a key factor for the exocytotic machinery in neuroendocrine cells.
Fiche publication
Date publication
mai 2001
Journal
The EMBO journal
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BADER Marie-France, Dr CHASSEROT-GOLAZ Sylvette, Dr VITALE Nicolas
Tous les auteurs :
Vitale N, Caumont AS, Chasserot-Golaz S, Du G, Wu S, Sciorra VA, Morris AJ, Frohman MA, Bader MF
Lien Pubmed
Résumé
Phospholipase D (PLD) has been proposed to mediate cytoskeletal remodeling and vesicular trafficking along the secretory pathway. We recently described the activation of an ADP ribosylation factor-regulated PLD at the plasma membrane of chromaffin cells undergoing secretagogue-stimulated exocytosis. We show here that the isoform involved is PLD1b, and, using a real-time assay for individual cells, that PLD activation and exocytosis are closely correlated. Moreover, overexpressed PLD1, but not PLD2, increases stimulated exocytosis in a phosphatidylinositol 4,5-bisphosphate-dependent manner, whereas catalytically inactive PLD1 inhibits it. These results provide the first direct evidence that PLD1 is an important component of the exocytotic machinery in neuroendocrine cells.
Mots clés
Actins, metabolism, Animals, Catalysis, Cattle, Cells, Cultured, Chromaffin Cells, cytology, Enzyme Inhibitors, pharmacology, Exocytosis, physiology, Intracellular Fluid, enzymology, Isoenzymes, antagonists & inhibitors, Neurosecretory Systems, cytology, PC12 Cells, Phosphatidylinositol 4,5-Diphosphate, metabolism, Phospholipase D, antagonists & inhibitors, Protein Kinase C, metabolism, Rats, Sphingosine, analogs & derivatives
Référence
EMBO J.. 2001 May;20(10):2424-34