Changes of skin collagen orientation associated with chronological aging as probed by polarized-FTIR micro-imaging.
Fiche publication
Date publication
mai 2014
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BRASSART-PASCO Sylvie, Pr PIOT Olivier
Tous les auteurs :
Nguyen TT, Eklouh-Molinier C, Sebiskveradze D, Feru J, Terryn C, Manfait M, Brassart-Pasco S, Piot O
Lien Pubmed
Résumé
During chronological skin aging, alterations in dermal structural proteins cause morphological modifications. Modifications are probably due to collagen fiber (type I collagen) rearrangement and reorientation with aging that have not been researched until now. FTIR microspectroscopy appears as an interesting method to study protein structure under normal and pathological conditions. Associated with a polarizer, this vibrational technique permits us to probe collagen orientation within skin tissue sections, by computing the ratio of integrated intensities of amide I and amide II bands. In this study, we used the polarized-FTIR imaging to evaluate molecular modifications of dermal collagen during chronological aging. The data processing of polarized infrared data revealed that type I collagen fibers become parallel to the skin surface in aged skin dermis. Our approach could find innovative applications in dermatology as well as in cosmetics.
Référence
Analyst. 2014 May 21;139(10):2482-8