Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA.
Fiche publication
Date publication
janvier 2017
Journal
Nature chemical biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEHM-ANSMANT Isabelle, Dr BRANLANT Christiane
Tous les auteurs :
Weldon C, Behm-Ansmant I, Hurley LH, Burley GA, Branlant C, Eperon IC, Dominguez C
Lien Pubmed
Résumé
RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
Référence
Nat. Chem. Biol.. 2017 Jan;13(1):18-20