mC Methylation Guides Systemic Transport of Messenger RNA over Graft Junctions in Plants.
Fiche publication
Date publication
juin 2019
Journal
Current biology : CB
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINLEIN Manfred
Tous les auteurs :
Yang L, Perrera V, Saplaoura E, Apelt F, Bahin M, Kramdi A, Olas J, Mueller-Roeber B, Sokolowska E, Zhang W, Li R, Pitzalis N, Heinlein M, Zhang S, Genovesio A, Colot V, Kragler F
Lien Pubmed
Résumé
In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcripts and how these may affect their mobility. We identified Arabidopsis thaliana mRNAs harboring the modified base 5-methylcytosine (mC) and found that these are significantly enriched in mRNAs previously described as mobile, moving over graft junctions to distinct plant parts. We confirm this finding with graft-mobile methylated mRNAs TRANSLATIONALLY CONTROLLED TUMOR PROTEIN 1 (TCTP1) and HEAT SHOCK COGNATE PROTEIN 70.1 (HSC70.1), whose mRNA transport is diminished in mutants deficient in mC mRNA methylation. Together, our results point toward an essential role of cytosine methylation in systemic mRNA mobility in plants and that TCTP1 mRNA mobility is required for its signaling function.
Mots clés
grafting, long-distance transport, m(5)C, mRNA methylation, mRNA modification, mRNA transport, phloem, plasmodesmata, root development
Référence
Curr. Biol.. 2019 Jun 29;: