Functional features of hemocyte subpopulations of the invasive mollusk species Dreissena polymorpha.
Fiche publication
Date publication
septembre 2016
Journal
Fish & shellfish immunology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr AUDONNET Sandra
Tous les auteurs :
Evariste L, Auffret M, Audonnet S, Geffard A, David E, Brousseau P, Fournier M, Betoulle S
Lien Pubmed
Résumé
Dreissena polymorpha is a mussel species that invaded many lotic and lentic inland waters in Western Europe and North America. Its positive or negative interactions with biotic and abiotic components of ecosystems are numerous, making this bivalve the subject of numerous studies in ecology, ecophysiology and ecotoxicology. In these contexts, the functional characterization of the zebra mussel hemocytes is of particular interest, as hemocytes are central cells involved in vital functions (immunity, growth, reproduction) of molluscan physiology. Dreissena polymorpha circulating hemocytes populations were characterized by a combination of structural and functional analysis. Assessments were performed during two contrasted physiological periods for mussels (gametogenesis and spawning). Three hemocyte types were identified as hyalinocytes and blast-like cells for agranular hemocytes and one granulocyte population. Flow cytometry analysis of hemocytes functionalities indicated that blast-like cells had low oxidative and mitochondrial activities and low lysosomal content. Hyalinocytes and granulocytes are fully equipped to perform innate immune response. Hyalinocytes exhibit higher oxidative activity than granulocytes. Such observation is not common since numerous studies show that granulocytes are usually cells that have the highest cellular activities. This result demonstrates the significant functional variability of hemocyte subpopulations. Moreover, our findings reveal that spawning period of Dreissena polymorpha was associated with an increase of hyalinocyte percentage in relation to low levels of biological activities in hemocytes. This reduction in hemocyte activity would reflect the important physiological changes associated with the spawning period of this invasive species known for its high reproductive potential.
Mots clés
Animals, Dreissena, cytology, France, Gametogenesis, Hemocytes, classification, Introduced Species, Reproduction, Seasons
Référence
Fish Shellfish Immunol.. 2016 Sep;56:144-154