Physical interactions between DNA and sepiolite nanofibers, and potential application for DNA transfer into mammalian cells.
Fiche publication
Date publication
novembre 2016
Journal
Scientific reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr PIETREMENT Olivier
Tous les auteurs :
Castro-Smirnov FA, Piétrement O, Aranda P, Bertrand JR, Ayache J, Le Cam E, Ruiz-Hitzky E, Lopez BS
Lien Pubmed
Résumé
Nanofibers of sepiolite, a natural silicate belonging to the clay minerals family, might constitute a potential promising nanocarrier for the non-viral transfer of bio-molecules. We show here that sepiolite nanofibers efficiently bind different types of DNA molecules through electrostatic interactions, hydrogen bonding, cation bridges, and van der Waals forces. Moreover, Fourier-transform infrared spectroscopy identified the external silanol groups as the main sites of interaction with the DNA. Furthermore, as a proof of concept, we show that sepiolite is able to stably transfer plasmid DNA into mammalian cells and that the efficiency can be optimized. Indeed, sonication of sepiolite 100-fold stimulated DNA transfection efficiency. These results open the way to the use of sepiolite-based biohybrids as a novel class of nanoplatform for gene transfer with potential clinical applications.
Mots clés
Animals, CHO Cells, Cell Line, Tumor, Cricetulus, DNA, metabolism, Gene Transfer Techniques, Hydrogen Bonding, Magnesium Silicates, metabolism, Nanofibers, Sonication, Spectroscopy, Fourier Transform Infrared, Static Electricity
Référence
Sci Rep. 2016 11 3;6:36341