Platelet lysate-based pro-angiogenic nanocoatings.
Fiche publication
Date publication
mars 2016
Journal
Acta biomaterialia
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MANO João F.
Tous les auteurs :
Oliveira SM, Pirraco RP, Marques AP, Santo VE, Gomes ME, Reis RL, Mano JF
Lien Pubmed
Résumé
Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides.
Mots clés
Angiogenesis, Endothelial cells, Growth factors, Instructive surfaces, Layer-by-layer assembling, Platelet lysate
Référence
Acta Biomater. 2016 Mar 1;32:129-137