Rapid antibiotic susceptibility testing on blood cultures using MALDI-TOF MS.
Fiche publication
Date publication
janvier 2018
Journal
PloS one
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HOCQUET Didier
Tous les auteurs :
Sauget M, Bertrand X, Hocquet D
Lien Pubmed
Résumé
Antibiotic resistance is a major public health problem requiring the early optimization of antibiotic prescriptions. Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry (MALDI-TOF MS) has been shown to accurately identify bacteria from positive blood culture. Here, we developed a rapid detection of Escherichia coli resistance to amoxicillin (AMX) and cefotaxime (CTX) from positive blood culture based on MALDI-TOF MS. Potential sparing of broad-spectrum antibiotics was also evaluated. We tested 103 E. coli-positive blood cultures. Blood cultures were subculture 1-hour in antibiotic-free rich liquid media before further incubation with and without AMX for 2.5 h or CTX for 2 h. Protein extracts associated with an internal control were spotted on a MALDI-TOF target and spectra were analyzed with the MBT-ASTRA prototype software (Bruker Daltonik GmbH, Bremen, Germany). Bacterial growth ratio was calculated from the AUC spectra obtained in the presence and absence of the antibiotic and compared to a threshold which classified E. coli as susceptible or resistant. Results were interpreted with MICs determined using agar dilution method as reference technique. MBT-ASTRA recognized 95% and 84% of the AMX- and CTX-susceptible isolates, respectively. Overall, quantitative analysis of mass spectra allows susceptibility testing within 4 hours after the positivity of blood culture with E. coli. At the first report of positive blood culture, MALDI-TOF MS would then provide the prescribers with the bacterial identification and the susceptibility to AMX and CTX, thus limiting the use of broad-spectrum compounds.
Mots clés
Amoxicillin, pharmacology, Anti-Bacterial Agents, pharmacology, Bacteriological Techniques, methods, Blood Culture, Cefotaxime, pharmacology, Drug Resistance, Bacterial, Escherichia coli, drug effects, Humans, Microbial Sensitivity Tests, methods, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, methods, Time Factors
Référence
PLoS ONE. 2018 ;13(10):e0205603