Abundant Extractable Metabolites from Temperate Tree Barks: The Specific Antimicrobial Activity of Extracts.
Fiche publication
Date publication
mars 2020
Journal
Antibiotics (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr NUZILLARD Jean-Marc
Tous les auteurs :
Abedini A, Colin M, Hubert J, Charpentier E, Angelis A, Bounasri H, Bertaux B, Kotland A, Reffuveille F, Nuzillard JM, Renault JH, Gangloff SC
Lien Pubmed
Résumé
Tree barks are mainly considered as wood wastes from forestry activities, but represent valuable resources as they may contain antimicrobial compounds. Here, we aimed to evaluate the possible antimicrobial activities of bark extracts and to characterize the chemical composition of the most active extract. Ten methanol bark extracts were tested in vitro against 17 bacterial strains and 5 yeast strains, through minimum inhibitory concentration (MIC) and minimum bactericidal (or fungicidal) concentration (MBC/MFC) assays. The extract from (E2-4) displayed the largest bactericidal activity against Gram-positive bacteria, with a lethal effect on 6 out of 8 strains. Antibiofilm assays of E2-4 were performed by crystal violet staining and enumeration of adhered bacteria. Assays demonstrated a biofilm inhibitory effect of E2-4 against CIP 53.154 at concentrations equal to or higher than 250 µg/mL. Chemical profiling of E2-4 by C nuclear magnetic resonance (NMR) revealed the presence of dihydrowogonin as a major constituent of the extract. E2-4 was fractionated by centrifugal partition chromatography and the three fractions containing dihydrowogonin were tested for their antibacterial and antibiofilm activities, revealing similar activities to those of E2-4. Dihydrowogonin was positively assessed as an interesting antimicrobial compound, which could be valued from wastes of barks.
Mots clés
13C nuclear magnetic resonance chemical profiling, Prunus avium, antibiofilm activity, antimicrobial activity, barks, centrifugal partition chromatography, dihydrowogonin, natural products
Référence
Antibiotics (Basel). 2020 Mar 4;9(3):