Some Plant Defense Stimulators can induce IL-1β production in human immune cells .
Fiche publication
Date publication
janvier 2020
Journal
Toxicology reports
Auteurs
Membres identifiés du Cancéropôle Est :
Pr CHLUBA Johanna
Tous les auteurs :
Teyssier L, Sorci G, Chluba J, Aimé S, Wendehenne D, Lamotte O, Connat JL
Lien Pubmed
Résumé
Among Plant Protection Products (PPP), a new emerging category of pesticides act by stimulating plant defense in order to improve plant resistance against microbial pathogens. Given that these compounds, the so-called Plant Defense Stimulators (PDS) act on innate immunity, we tested, using an approach on human mononuclear leucocytes (PBMC), the potential toxicity (XTT assay) and inflammatory effects (production of IL-1β) of 4 PPP belonging to different chemical families. We found that two products (LBG-01F34® and Regalis®) did not induce any cytotoxicity or IL-1 β production. The product BION-50 WG®, that contains Acibenzolar-S-methyl (ASM) and silica particles did not present any cytotoxicity but induced a significant increase in the production of the inflammatory cytokine IL-1 β. Finally, Vacciplant® that contains laminarin, was highly cytotoxic and pro-inflammatory. It induced a strong production of IL-1 β when used at a concentration in the culture medium, as low as 0.02 mg/mL. We also tested the potential toxic effect of these 4 PPP on 4 days old zebra fish larvae. After 24 h of exposure, our results indicate that Vacciplant® induced zebra fish larvae mortality at concentration of 20 μg/mL. LBG did not induced significant mortality at concentrations up to 1 mg/mL whereas Regalis was lethal for 0,3 mg/mL concentrations and BION-50 WG began to induce mortality at 2,5 mg/mL. Our results indicate possible effects of PDS on IL-1β production in human cells and fish survival, calling for more studies on the potential noxious side effects of these compounds.
Mots clés
ASM, acyl-benzolar-S-methyl, Cell metabolic activity, Danio rerio, IL-1β, Inflammation, PBMC, PBMC, peripheral blood mononuclear cells, PDS, Plant Defense Stimulator, PPP, Plant Protection product, Pesticides, Plant Defense Stimulator, XTT, Zebra fish
Référence
Toxicol Rep. 2020 ;7:413-420