Effect of haemolysis on FTIR and Raman spectra of blood plasma.
Fiche publication
Date publication
mars 2020
Journal
Journal of biophotonics
Auteurs
Membres identifiés du Cancéropôle Est :
Pr SOCKALINGUM Ganesh
Tous les auteurs :
Medipally DKR, Cullen D, Untereiner V, Bryant J, Sockalingum GD, Nguyen TNQ, Noone E, Bradshaw S, Finn M, Dunne M, Shannon AM, Armstrong J, Meade AD, Lyng FM
Lien Pubmed
Résumé
Haemolysis is a very common phenomenon and is referred as the release of intracellular components from red blood cells to the extracellular fluid. Haemolysed samples are often rejected in clinics due to the interference of haemoglobin and intracellular components in laboratory measurements. Plasma and serum based vibrational spectroscopy studies are extensively applied to generate spectral biomarkers for various diseases. However, no studies have reported the effect of haemolysis in blood based vibrational spectroscopy studies. This study was undertaken to evaluate the effect of haemolysis on infrared and Raman spectra of blood plasma. In this study, prostate cancer plasma samples (n = 30) were divided into three groups (non-haemolysed, mildly haemolysed and moderately hemolysed) based on the degree of haemolysis and FTIR and Raman spectra were recorded using high throughput (HT)-FTIR and HT-Raman spectroscopy. Discrimination was observed between the infrared and Raman spectra of non-haemolysed and haemolysed plasma samples using principal component analysis. A classical least square fitting analysis showed differences in the weighing of pure components in non-haemolysed and haemolysed plasma samples. Therefore, it is worth to consider the changes in spectral features due to haemolysis when comparing the results within and between experiments. This article is protected by copyright. All rights reserved.
Mots clés
FTIR spectroscopy, Haemolysis, Raman spectroscopy, blood plasma, classical least squares fitting analysis, principal component analysis
Référence
J Biophotonics. 2020 Mar 12;:e2455