Brillouin spectroscopy: a new tool to decipher viscoelastic properties of biological scaffold functionalized with nanoscale films.
Fiche publication
Date publication
janvier 2013
Journal
Bio-medical materials and engineering
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MENU Patrick, Dr ROUXEL Didier, Dr KERDJOUDJ Halima
Tous les auteurs :
Beroud J, Vincent B, Paternotte E, Nguyen VS, Kerdjoudj H, Velot E, Rouxel D, Menu P
Lien Pubmed
Résumé
In tissue engineering, the endothelialization of vascular scaffold can be a crucial step to improve graft patency. A functional cellularization requires coating surfaces. Since 2003, our group used polyelectrolyte multilayer films (PEMFs) made of poly(allylamine hydrochloride) and polystyren sulfonate to coat luminal surface of blood vessel. Previous results showed that PEMFs have remarkable effect on cellular behavior: adhesion, proliferation, differentiation. However, no method seems adapted for in vitro measurement of the viscoelastic shift after PEMFs buildup.
Mots clés
Algorithms, Animals, Carotid Arteries, anatomy & histology, Chelating Agents, Coated Materials, Biocompatible, chemistry, Elasticity, Endothelial Cells, cytology, Endothelium, Vascular, anatomy & histology, Femoral Artery, anatomy & histology, Fluorescent Dyes, Humans, Lasers, Male, Microscopy, Confocal, Nanostructures, chemistry, Phonons, Photoacoustic Techniques, Photons, Polyamines, chemistry, Polystyrenes, Rabbits, Scattering, Radiation, Spectrum Analysis, methods, Tissue Scaffolds, chemistry, Tunica Intima, anatomy & histology, Umbilical Arteries, anatomy & histology, Venae Cavae, anatomy & histology, Viscosity
Référence
Biomed Mater Eng. 2013 ;23(4):251-61