Claudin 1 inhibits cell migration and increases intercellular adhesion in triple-negative breast cancer cell line.
Fiche publication
Date publication
septembre 2020
Journal
Molecular biology reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GRILLIER-VUISSOZ Isabelle, Dr KUNTZ Sandra
Tous les auteurs :
Geoffroy M, Kleinclauss A, Kuntz S, Grillier-Vuissoz I
Lien Pubmed
Résumé
Triple-negative "claudin 1 low" subtype represents around 15% of breast cancer and displays poor prognosis. The loss of claudin 1 is correlated with increased invasiveness and higher recurrence of the disease. Claudin 1 constitutes the backbone of the tight junction and is involved in cell-cell adhesion and migration processes. However, studies showed a controversial role of claudin 1 in cell migration. In this study, we aimed to clarify the effect of claudin 1 on migration of mesenchymal triple-negative breast cancer cells (TNBC). We reported that transient over expression of claudin 1 in MDA-MB-231 and Hs578T "claudin 1 low" TNBC cells inhibited cell migration using wound healing and transwell migration assays. In order to investigate more specifically the involvement of claudin 1, we generated stable MDA-MB-231 clones overexpressing claudin 1. Interestingly, the level of claudin 1 was correlated to the inhibition of cell migration and to the increase of cell-cell aggregation associated with enhanced formation of β-catenin adherens junction and occludin tight junction. Finally, we reported for the first time the key role of claudin 1 in the inhibition of cell migration process associated with the disappearance of stress fibers. These data suggest that re-expression of claudin 1 could be a promising strategy for regulating the migration of TNBC which no longer express claudin 1.
Mots clés
Cell junctions, Claudin 1, Migration, Stress fiber, Triple negative breast cancer
Référence
Mol. Biol. Rep.. 2020 Sep 26;: