Unexpected high production of biohydrogen from the endogenous fermentation of grape must deposits.
Fiche publication
Date publication
octobre 2020
Journal
Bioresource technology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr ERNST Barbara
Tous les auteurs :
François E, Dumas C, Gougeon RD, Alexandre H, Vuilleumier S, Ernst B
Lien Pubmed
Résumé
The aim of this work was to assess the performances of wine byproduct biomass for hydrogen production by dark fermentation. Grape must deposits from two grape varieties (Pinot Gris and Chardonnay) were considered, either with external microbial inoculum or without. We show that grape must residues contain endogenous microflora, well adapted to their environment, which can degrade sugars (initially contained in the biomass) to hydrogen without any nutrient addition. Indeed, hydrogen production during endogenous fermentation is as efficient as with an external heat-treated inoculum (2.5 ± 0.4 L.L and 1.61 ± 0.41 mol.mol, respectively) with a lower energy cost. Hydrogen-producing bacteria were selected from the endogenous microflora during semi-batch bioreactor operation, as shown by T-RFLP profiles and 16S rRNA sequencing, with Clostridium spp. (butyricum, beijerinckii, diolis, roseum) identified as the major phylotype. Such hydrogen production efficiency opens new perspectives for innovating in the valorization of winery by-products.
Mots clés
Dark fermentation, Endogenous fermentation, Hydrogen, Valorization, Winery waste
Référence
Bioresour Technol. 2020 Oct 29;320(Pt A):124334