Dual Gene Delivery Reagents From Antiproliferative Alkylphospholipids for Combined Antitumor Therapy.

Fiche publication


Date publication

janvier 2020

Journal

Frontiers in chemistry

Auteurs

Membres identifiés du Cancéropôle Est :
Dr LEBEAU Luc, Dr REMY Jean-Serge, Pr PONS Françoise


Tous les auteurs :
Gaillard B, Remy JS, Pons F, Lebeau L

Résumé

Alkylphospholipids (APLs) have elicited great interest as antitumor agents due to their unique mode of action on cell membranes. However, their clinical applications have been limited so far by high hemolytic activity. Recently, cationic prodrugs of erufosine, a most promising APL, have been shown to mediate efficient intracellular gene delivery, while preserving the antiproliferative properties of the parent APL. Here, cationic prodrugs of the two APLs that are currently used in the clinic, miltefosine, and perifosine, are investigated and compared to the erufosine prodrugs. Their synthesis, stability, gene delivery and self-assembly properties, and hemolytic activity are discussed in detail. Finally, the potential of the pro-miltefosine and pro-perifosine compounds and in combined antitumor therapy is demonstrated using pUNO1-hTRAIL, a plasmid DNA encoding TRAIL, a member of the TNF superfamily. With these pro-APL compounds, we provide a proof of concept for a new promising strategy for cancer therapy combining gene therapy and APL-based chemotherapy.

Mots clés

alkylphospholipid, chemotherapy, erufosine, gene therapy, hemolytic toxicity, miltefosine, perifosine, prodrug

Référence

Front Chem. 2020 ;8:581260