Increased internalization of Staphylococcus aureus and cytokine expression in human Wharton's jelly mesenchymal stem cells.
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Date publication
janvier 2014
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GANGLOFF Sophie, Dr VELARD Frédéric, Dr KERDJOUDJ Halima
Tous les auteurs :
Josse J, Velard F, Mechiche Alami S, Brun V, Guillaume C, Kerdjoudj H, Lamkhioued B, Gangloff SC
Lien Pubmed
Résumé
BACKGROUND: Although a large number of studies have documented the interaction of mesenchymal stem cells (MSCs) with cells of both the innate and adaptive immune systems, not much is known about how bacteria interact with MSCs and how this might influence MSCs behavior. In this study, we investigated the impact of Staphylococcus aureus (S. aureus), on viability and cytokines' production of human Wharton's jelly-MSCs (WJ-MSCs). OBJECTIVE: To investigate if WJ-MSCs: (1) internalize S. aureus; (2) are able to survive and (3) release immunomodulatory mediators after interaction with S. aureus. METHODS: WJ-MSCs were exposed to S. aureus at a multiplicity of infection (MOI) of 10:1 or 30:1 for different designed times. After interaction, intracellular bacteria were quantified as well as MSCs viability. Expression and cytokine-secretion were assessed using quantitative real-time PCR and ELISA. RESULTS: We found that the challenge of WJ-MSCs with S. aureus resulted in increased internalization of S. aureus in a time-dependent manner until six hours post-infection at either MOI of 10:1 and 30:1 and in increased expression of IL-6 mRNA and secretion of TNF-alpha at six hours and nine hours post-infection (p
Référence
Biomed Mater Eng. 2014;24(1 Suppl):27-35