Two routes to senescence revealed by real-time analysis of telomerase-negative single lineages.
Fiche publication
Date publication
juillet 2015
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHARVIN Gilles
Tous les auteurs :
Xu Z, Fallet E, Paoletti C, Fehrmann S, Charvin G, Teixeira MT
Lien Pubmed
Résumé
In eukaryotes, telomeres cap chromosome ends to maintain genomic stability. Failure to maintain telomeres leads to their progressive erosion and eventually triggers replicative senescence, a pathway that protects against unrestricted cell proliferation. However, the mechanisms underlying the variability and dynamics of this pathway are still elusive. Here we use a microfluidics-based live-cell imaging assay to investigate replicative senescence in individual Saccharomyces cerevisiae cell lineages following telomerase inactivation. We characterize two mechanistically distinct routes to senescence. Most lineages undergo an abrupt and irreversible switch from a replicative to an arrested state, consistent with telomeres reaching a critically short length. In contrast, other lineages experience frequent and stochastic reversible arrests, consistent with the repair of accidental telomere damage by Pol32, a subunit of polymerase δ required for break-induced replication and for post-senescence survival. Thus, at the single-cell level, replicative senescence comprises both deterministic cell fates and chaotic cell division dynamics.
Mots clés
Blotting, Southern, Cell Cycle Checkpoints, Cell Division, Cell Lineage, DNA Breaks, DNA Repair, DNA-Directed DNA Polymerase, metabolism, Lab-On-A-Chip Devices, Microscopy, Fluorescence, Microscopy, Phase-Contrast, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, genetics, Telomerase, genetics, Telomere, metabolism, Telomere Shortening, Time-Lapse Imaging
Référence
Nat Commun. 2015 Jul 9;6:7680