Visualization of RNA-Quadruplexes in Live Cells.
Fiche publication
Date publication
juillet 2015
Journal
Journal of the American Chemical Society
Auteurs
Membres identifiés du Cancéropôle Est :
Pr PERRIER-CORNET Jean-Marie, Dr MONCHAUD David
Tous les auteurs :
Laguerre A, Hukezalie K, Winckler P, Katranji F, Chanteloup G, Pirrotta M, Perrier-Cornet JM, Wong JM, Monchaud D
Lien Pubmed
Résumé
Visualization of DNA and RNA quadruplex formation in human cells was demonstrated recently with different quadruplex-specific antibodies. Despite the significant interest in these immunodetection approaches, dynamic detection of quadruplex in live cells remains elusive. Here, we report on NaphthoTASQ (N-TASQ), a next-generation quadruplex ligand that acts as a multiphoton turn-on fluorescent probe. Single-step incubation of human and mouse cells with N-TASQ enables the direct detection of RNA-quadruplexes in untreated cells (no fixation, permeabilization or mounting steps), thus offering a unique, unbiased visualization of quadruplexes in live cells.
Mots clés
Animals, Biomimetics, Cations, Cell Line, Tumor, Chelating Agents, chemistry, DNA, genetics, Fluorescence Resonance Energy Transfer, Fluorescent Dyes, chemistry, G-Quadruplexes, Humans, Ligands, MCF-7 Cells, Melanoma, Experimental, Mice, Microscopy, Fluorescence, methods, Photons, RNA, chemistry, Static Electricity
Référence
J. Am. Chem. Soc.. 2015 Jul 8;137(26):8521-5