Opportunistic use of catecholamine neurotransmitters as siderophores to access iron by Pseudomonas aeruginosa.
Fiche publication
Date publication
décembre 2020
Journal
Environmental microbiology
Auteurs
Membres identifiés du Cancéropôle Est :
Mr HAMMANN Philippe
Tous les auteurs :
Perraud Q, Kuhn L, Fritsch S, Graulier G, Gasser V, Normant V, Hammann P, Schalk IJ
Lien Pubmed
Résumé
Iron is an essential nutrient for bacterial growth and the cause of a fierce battle between the pathogen and host during infection. Bacteria have developed several strategies to access iron from the host, the most common being the production of siderophores, small iron-chelating molecules secreted into the bacterial environment. The opportunist pathogen Pseudomonas aeruginosa produces two siderophores, pyoverdine and pyochelin, and is also able to use a wide panoply of xenosiderophores, siderophores produced by other microorganisms. Here, we demonstrate that catecholamine neurotransmitters (dopamine, L-DOPA, epinephrine, and norepinephrine) are able to chelate iron and efficiently bring iron into P. aeruginosa cells via TonB-dependent transporters (TBDTs). Bacterial growth assays under strong iron-restricted conditions and with numerous mutants showed that the TBDTs involved are PiuA and PirA. PiuA exhibited more pronounced specificity for dopamine uptake than for norepinephrine, epinephrine, and L-DOPA, whereas PirA specificity appeared to be higher for L-DOPA and norepinephrine. Proteomic and qRT-PCR approaches showed pirA transcription and expression to be induced in the presence of all four catecholamines. Finally, the oxidative properties of catecholamines enable them to reduce iron, and we observed ferrous iron uptake via the FeoABC system in the presence of L-DOPA. This article is protected by copyright. All rights reserved.
Référence
Environ Microbiol. 2020 Dec 21;: