Microfluidics for single-cell lineage tracking over time to characterize transmission of phenotypes in .
Fiche publication
Date publication
décembre 2020
Journal
STAR protocols
Auteurs
Membres identifiés du Cancéropôle Est :
Dr CHARVIN Gilles
Tous les auteurs :
Bheda P, Aguilar-Gómez D, Kukhtevich I, Becker J, Charvin G, Kirmizis A, Schneider R
Lien Pubmed
Résumé
The budding yeast is an excellent model organism to dissect the maintenance and inheritance of phenotypes due to its asymmetric division. This requires following individual cells over time as they go through divisions to define pedigrees. Here, we provide a detailed protocol for collecting and analyzing time-lapse imaging data of yeast cells. The microfluidics protocol can achieve improved time resolution for single-cell tracking to enable characterization of maintenance and inheritance of phenotypes. For complete details on the use and execution of this protocol, please refer to Bheda et al. (2020a).
Mots clés
Cell biology, Microscopy, Model organisms, Single cell
Référence
STAR Protoc. 2020 Dec 18;1(3):100228