Mapping rRNA 2'-O-methylations and identification of C/D snoRNAs in Arabidopsis thaliana plants.
Fiche publication
Date publication
février 2021
Journal
RNA biology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MOTORINE Iouri, Dr MARCHAND Virginie, Dr CARAPITO Christine
Tous les auteurs :
Azevedo-Favory J, Gaspin C, Ayadi L, Montacié C, Marchand V, Jobet E, Rompais M, Carapito C, Motorin Y, Sáez-Vásquez J
Lien Pubmed
Résumé
In all eukaryotic cells, the most abundant modification of ribosomal RNA (rRNA) is methylation at the ribose moiety (2'--methylation). Ribose methylation at specific rRNA sites is guided by small nucleolar RNAs (snoRNAs) of C/D-box type (C/D snoRNA) and achieved by the methyltransferase Fibrillarin (FIB). Here we used the Illumina-based RiboMethSeq approach for mapping rRNA 2'--methylation sites in Col-0 (WT) plants. This analysis detected novel C/D snoRNA-guided rRNA 2'--methylation positions and also some orphan sites without a matching C/D snoRNA. Furthermore, immunoprecipitation of Arabidopsis FIB2 identified and demonstrated expression of C/D snoRNAs corresponding to majority of mapped rRNA sites. On the other hand, we show that disruption of Arabidopsis Nucleolin 1 gene (NUC1), encoding a major nucleolar protein, decreases 2'--methylation at specific rRNA sites suggesting functional/structural interconnections of 2'--methylation with nucleolus organization and plant development. Finally, based on our findings and existent database sets, we introduce a new nomenclature system for C/D snoRNA in Arabidopsis plants.
Mots clés
2ʹ-O-Methylation, C/D snoRNA, arabidopsis, fibrillarin, nucleolin, rRNA
Référence
RNA Biol. 2021 Feb 17;:1-18