Validation of a Three-Dimensional Head and Neck Spheroid Model to Evaluate Cameras for NIR Fluorescence-Guided Cancer Surgery.
Fiche publication
Date publication
février 2021
Journal
International journal of molecular sciences
Auteurs
Membres identifiés du Cancéropôle Est :
Dr BEZDETNAYA-BOLOTINE Lina, Pr DOLIVET Gilles, Dr LASSALLE Henri-Pierre
Tous les auteurs :
Egloff-Juras C, Yakavets I, Scherrer V, Francois A, Bezdetnaya L, Lassalle HP, Dolivet G
Lien Pubmed
Résumé
Near-infrared (NIR) fluorescence-guided surgery is an innovative technique for the real-time visualization of resection margins. The aim of this study was to develop a head and neck multicellular tumor spheroid model and to explore the possibilities offered by it for the evaluation of cameras for NIR fluorescence-guided surgery protocols. FaDu spheroids were incubated with indocyanine green (ICG) and then included in a tissue-like phantom. To assess the capability of Fluobeam® NIR camera to detect ICG in tissues, FaDu spheroids exposed to ICG were embedded in 2, 5 or 8 mm of tissue-like phantom. The fluorescence signal was significantly higher between 2, 5 and 8 mm of depth for spheroids treated with more than 5 µg/mL ICG ( < 0.05). The fluorescence intensity positively correlated with the size of spheroids ( < 0.01), while the correlation with depth in the tissue-like phantom was strongly negative ( < 0.001). This multicellular spheroid model embedded in a tissue-like phantom seems to be a simple and reproducible in vitro tumor model, allowing a comparison of NIR cameras. The ideal configuration seems to be 450 μm FaDu spheroids incubated for 24 hours with 0.05 mg/ml of ICG, ensuring the best stability, toxicity, incorporation and signal intensity.
Mots clés
fluorescence-guided surgery, head and neck cancer, indocyanine green, multicellular tumor spheroids
Référence
Int J Mol Sci. 2021 Feb 17;22(4):