Multimodal Approach for Cancer Cell Investigation.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DEVY Jérôme, Dr BERQUAND Alexandre
Tous les auteurs :
Berquand A, Devy J
Lien Pubmed
Résumé
Atomic force microscopy (AFM) enables the characterization of a wide range of samples including live cells. It is generally admitted that cancer cells are significantly softer than their normal counterparts, but imaging live cells by AFM using traditional modes can be at the cost of time or resolution. We describe how this tool can be used to estimate the motility of cancer versus normal cells, based on topographical and mechanical approaches, and coupled to optical imaging.
Mots clés
Atomic force microscopy, Cancer, Fluorescence imaging, Force measurements, PeakForce QNM, Video microscopy
Référence
Methods Mol Biol. 2021 ;2350:289-297