Unravelling the Puzzle of Anthranoid Metabolism in Living Plant Cells Using Spectral Imaging Coupled to Mass Spectrometry.
Fiche publication
Date publication
août 2021
Journal
Metabolites
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MUTTERER Jérôme, Dr ELHABIRI Mourad
Tous les auteurs :
Chevalier Q, Gallé JB, Wasser N, Mazan V, Villette C, Mutterer J, Elustondo MM, Girard N, Elhabiri M, Schaller H, Hemmerlin A, Vonthron-Sénécheau C
Lien Pubmed
Résumé
Vismione H (VH) is a fluorescent prenylated anthranoid produced by plants from the Hypericaceae family, with antiprotozoal activities against malaria and leishmaniosis. Little is known about its biosynthesis and metabolism in plants or its mode of action against parasites. When VH is isolated from , it is rapidly converted into madagascine anthrone and anthraquinone, which are characterized by markedly different fluorescent properties. To locate the fluorescence of VH in living plant cells and discriminate it from that of the other metabolites, an original strategy combining spectral imaging (SImaging), confocal microscopy, and non-targeted metabolomics using mass spectrometry, was developed. Besides VH, structurally related molecules including madagascine (Mad), emodin (Emo), quinizarin (Qui), as well as lapachol (Lap) and fraxetin (Fra) were analyzed. This strategy readily allowed a spatiotemporal characterization and discrimination of spectral fingerprints from anthranoid-derived metabolites and related complexes with cations and proteins. In addition, our study validates the ability of plant cells to metabolize VH into madagascine anthrone, anthraquinones and unexpected metabolites. These results pave the way for new hypotheses on anthranoid metabolism in plants.
Mots clés
anthranoids, antimalarial drugs, mass spectrometry, metabolism, spectral imaging
Référence
Metabolites. 2021 Aug 25;11(9):