Higher-energy collision-induced dissociation for the quantification by liquid chromatography/tandem ion trap mass spectrometry of nitric oxide metabolites coming from S-nitroso-glutathione in an in vitro model of the intestinal barrier.
Fiche publication
Date publication
janvier 2019
Journal
Rapid communications in mass spectrometry : RCM
Auteurs
Membres identifiés du Cancéropôle Est :
Dr VERNEX-LOSET Lionel
Tous les auteurs :
Yu H, Bonetti J, Gaucher C, Fries I, Vernex-Loset L, Leroy P, Chaimbault P
Lien Pubmed
Résumé
The potency of S-nitrosoglutathione (GSNO) as a nitric oxide (NO) donor to treat cardiovascular diseases (CVDs) has been highlighted in numerous studies. In order to study its bioavailability after oral administration, which represents the most convenient route for the chronic treatment of CVDs, it is essential to develop an analytical method permitting (i) the simultaneous measurement of GSNO metabolites, i.e. nitrite, S-nitrosothiols (RSNOs) and nitrate and (ii) to distinguish them from other sources (endogenous synthesis and diet).
Mots clés
2-Naphthylamine, analogs & derivatives, Caco-2 Cells, Chromatography, Liquid, methods, Humans, Intestinal Absorption, drug effects, Limit of Detection, Nitric Oxide, analysis, Nitrites, chemistry, Reproducibility of Results, S-Nitrosoglutathione, metabolism, Tandem Mass Spectrometry, instrumentation
Référence
Rapid Commun Mass Spectrom. 2019 Jan 15;33(1):1-11