Detection of Infectious Oocysts from Lamb's Lettuce: CC-qPCR's Intake.
Fiche publication
Date publication
janvier 2021
Journal
Microorganisms
Auteurs
Membres identifiés du Cancéropôle Est :
Pr VILLENA Isabelle
Tous les auteurs :
Kubina S, Costa D, Favennec L, Gargala G, Rousseau A, Villena I, La Carbona S, Razakandrainibe R
Lien Pubmed
Résumé
spp. is responsible for several food and waterborne disease outbreaks worldwide. Healthier lifestyles attract consumers to eat, notably, fresh food like fruits and vegetables. The consumption of raw or under-cooked food increases the risk of foodborne transmission of Cryptosporidiosis. The assessment of the consumer's exposure to danger is crucial for public health. Still, the standardized method to detect this parasite in fresh leafy greens and berry fruits has only been available since 2016 and suffers from weaknesses. Consequently, in this study, we propose a method with minimum processing steps that combines cell culture and the quantitative PCR (CC-qPCR) for detecting infectious oocysts recovered from lamb's lettuce. This CC-qPCR is a rapid and easy method that can detect up to one oocyst, whereas it is undetectable by classic qPCR.
Mots clés
Cryptosporidium, detection, fresh vegetables, in vitro method, oocysts infectivity
Référence
Microorganisms. 2021 Jan 21;9(2):