In Vivo Aniline Blue Staining and Semiautomated Quantification of Callose Deposition at Plasmodesmata.
Fiche publication
Date publication
janvier 2022
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MUTTERER Jérôme, Dr HEINLEIN Manfred
Tous les auteurs :
Huang C, Mutterer J, Heinlein M
Lien Pubmed
Résumé
The deposition and turnover of callose (beta-1,3 glucan polymer) in the cell wall surrounding the neck regions of plasmodesmata (PD) controls the cell-to-cell diffusion rate of molecules and, therefore, plays an important role in the regulation of intercellular communication in plants.Here we describe a simple and fast in vivo staining procedure for the imaging and quantification of callose at PD. We also introduce calloseQuant, a plug-in for semiautomated image analysis and non-biased quantification of callose levels at PD using ImageJ.
Mots clés
Aniline blue, Callose, Confocal microscopy, ImageJ, Plasmodesmata, flg22
Référence
Methods Mol Biol. 2022 ;2457:151-165