Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability.
Fiche publication
Date publication
janvier 2022
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr HEINLEIN Manfred
Tous les auteurs :
Lazareva EA, Atabekova AK, Lezzhov AA, Morozov SY, Heinlein M, Solovyev AG
Lien Pubmed
Résumé
Plant virus movement proteins (MPs) mediate cell-to-cell movement of the virus genome through plasmodesmata (PD). MPs target PD to increase their size exclusion limit (SEL), and this MP function is essential for virus intercellular trafficking. In this chapter, we describe the use of a Potato virus X genome-derived reporter for agroinfiltration-based identification of virus genome-encoded MPs and analysis of the ability of individual viral MPs or plant proteins to increase the PD SEL.
Mots clés
Cell-to-cell movement, Movement protein, Plant virus, Plasmodesmata, Size exclusion limit
Référence
Methods Mol Biol. 2022 ;2457:333-349