Aerosol-Cell Exposure System Applied to Semi-Adherent Cells for Aerosolization of Lung Surfactant and Nanoparticles Followed by High Quality RNA Extraction.
Fiche publication
Date publication
avril 2022
Journal
Nanomaterials (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr RIHN Bertrand
Tous les auteurs :
Leroux MM, Hocquel R, Bourge K, Kokot B, Kokot H, Koklič T, Štrancar J, Ding Y, Kumar P, Schmid O, Rihn BH, Ferrari L, Joubert O
Lien Pubmed
Résumé
Nanoparticle toxicity assessments have moved closer to physiological conditions while trying to avoid the use of animal models. An example of new in vitro exposure techniques developed is the exposure of cultured cells at the air-liquid interface (ALI), particularly in the case of respiratory airways. While the commercially available VITROCELL Cloud System has been applied for the delivery of aerosolized substances to adherent cells under ALI conditions, it has not yet been tested on lung surfactant and semi-adherent cells such as alveolar macrophages, which are playing a pivotal role in the nanoparticle-induced immune response.
Mots clés
NR8383, RNA extraction, VITROCELL® Cloud System, air–liquid interface, macrophages, nanoparticles, surfactant, transcriptomic
Référence
Nanomaterials (Basel). 2022 Apr 15;12(8):