Umbilical Cord Blood as a Source of Less Differentiated T Cells to Produce CD123 CAR-T Cells.
Fiche publication
Date publication
juin 2022
Journal
Cancers
Auteurs
Membres identifiés du Cancéropôle Est :
Pr ADOTEVI Olivier, Pr GARNACHE-OTTOU Francine, Dr GODET Yann, Dr GALAINE Jeanne, Dr POUTHIER Fabienne, Dr ANGELOT-DELETTRE Fanny
Tous les auteurs :
Caël B, Galaine J, Bardey I, Marton C, Fredon M, Biichle S, Poussard M, Godet Y, Angelot-Delettre F, Barisien C, Bésiers C, Adotevi O, Pouthier F, Garnache-Ottou F, Bôle-Richard E
Lien Pubmed
Résumé
Chimeric Antigen Receptor (CAR) therapy has led to great successes in patients with leukemia and lymphoma. Umbilical Cord Blood (UCB), stored in UCB banks, is an attractive source of T cells for CAR-T production. We used a third generation CD123 CAR-T (CD28/4-1BB), which was previously developed using an adult's Peripheral Blood (PB), to test the ability of obtaining CD123 CAR-T from fresh or cryopreserved UCB. We obtained a cell product with a high and stable transduction efficacy, and a poorly differentiated phenotype of CAR-T cells, while retaining high cytotoxic functions in vitro and in vivo. Moreover, CAR-T produced from cryopreserved UCB are as functional as CAR-T produced from fresh UCB. Overall, these data pave the way for the clinical development of UCB-derived CAR-T. UCB CAR-T could be transferred in an autologous manner (after an UCB transplant) to reduce post-transplant relapses, or in an allogeneic setting, thanks to fewer HLA restrictions which ease the requirements for a match between the donor and recipient.
Mots clés
CAR-T cells, CD123, Umbilical Cord Blood, adoptive cell therapy, allogeneic immunotherapy
Référence
Cancers (Basel). 2022 06 28;14(13):