Alcoholic precipitation of small non-coding RNAs.
Fiche publication
Date publication
janvier 2015
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr REDERSTORFF Mathieu
Tous les auteurs :
Clerget G, Bourguignon-Igel V, Rederstorff M
Lien Pubmed
Résumé
Alcoholic precipitation is a critical step to recover RNA of high purity. This chapter describes the principles of alcoholic precipitation as well as a standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.
Mots clés
2-Propanol, chemistry, Chemical Precipitation, drug effects, Chemistry Techniques, Analytical, methods, Ethanol, chemistry, RNA, Small Untranslated, chemistry
Référence
Methods Mol. Biol.. 2015 ;1296:11-6