TCR signaling events are required for maintaining CD4 regulatory T cell numbers and suppressive capacities in the periphery.

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Date publication

décembre 2014

Journal

Journal of immunology (Baltimore, Md. : 1950)

Auteurs

Membres identifiés du Cancéropôle Est :
Dr CHARVET Céline, Dr POMMIER Arnaud


Tous les auteurs :
Delpoux A, Yakonowsky P, Durand A, Charvet C, Valente M, Pommier A, Bonilla N, Martin B, Auffray C, Lucas B

Résumé

CD4 regulatory T cells (Tregs) can be subdivided into two subsets according to Ly-6C expression in the periphery. Phenotypic analysis, imaging, and adoptive-transfer experiments of peripheral Ly-6C(-) and Ly-6C(+) Tregs reveal that the nonexpression of Ly-6C by ∼70% of peripheral Tregs depends on TCR signaling events. Interestingly, Ly-6C(-) Tregs express higher surface amounts of key immunosuppressive molecules than do Ly-6C(+) Tregs and produce constitutively anti-inflammatory cytokines. In line with their phenotype, Ly-6C(+) Tregs exhibit poor suppressive capacities in vitro and in vivo. Finally, although Ly-6C(-) Tregs maintain their numbers with age, Ly-6C(+) Tregs gradually disappear. Altogether, our data strongly suggest that both the survival and suppressive functions of peripheral CD4 Tregs rely on their ability to receive strong TCR signals.

Mots clés

Age Factors, Aging, immunology, Animals, Antigens, Ly, genetics, Antigens, Surface, genetics, Gene Expression, Immunomodulation, Immunophenotyping, Lymph Nodes, immunology, Lymphocyte Activation, genetics, Mice, Mice, Transgenic, Phenotype, Receptors, Antigen, T-Cell, metabolism, Signal Transduction, T-Lymphocyte Subsets, immunology, T-Lymphocytes, Regulatory, immunology

Référence

J. Immunol.. 2014 Dec 15;193(12):5914-23