Questions about Residual Cell Viability in Cryopreserved Human Amniotic Membrane and Its Impact on Clinical Applications.
Fiche publication
Date publication
octobre 2022
Journal
Biomedicines
Auteurs
Membres identifiés du Cancéropôle Est :
Dr GINDRAUX Florelle
Tous les auteurs :
Gaudet C, Solecki L, Mathéaud B, Odet S, Meyer C, Louvrier A, Gindraux F
Lien Pubmed
Résumé
We questioned the relevance of evaluating residual cell viability in human amniotic membrane (hAM) after its cryopreservation since cell survival is controversial and its ability to act as a matrix (including the presence of growth factors and cytokines) appears to be most important for tissue regeneration purposes. We also discussed the usefulness of osteodifferentiating amniotic cells in whole hAM for bone repair applications. We have evidence that determining residual cell viability after cryopreservation and hAM osteodifferentiation is not justified.
Mots clés
amniotic membrane, cell survival, cryocongelation, cryopreservation, differentiation, matrix, quality controls, viability
Référence
Biomedicines. 2022 10 1;10(10):