Medium throughput bisulfite sequencing for accurate detection of 5-methylcytosine and 5-hydroxymethylcytosine.
Fiche publication
Date publication
janvier 2017
Journal
BMC genomics
Auteurs
Membres identifiés du Cancéropôle Est :
Dr LUTZ Pierre-Eric
Tous les auteurs :
Chen GG, Gross JA, Lutz PE, Vaillancourt K, Maussion G, Bramoulle A, Théroux JF, Gardini ES, Ehlert U, Bourret G, Masurel A, Lepage P, Mechawar N, Turecki G, Ernst C
Lien Pubmed
Résumé
Epigenetic modifications of DNA, such as 5-methylcytosine and 5-hydroxymethycytosine, play important roles in development and disease. Here, we present a cost-effective and versatile methodology for the analysis of DNA methylation in targeted genomic regions, which comprises multiplexed, PCR-based preparation of bisulfite DNA libraries followed by customized MiSeq sequencing.
Mots clés
5-hydroxymethylcytosine, 5-methylcytosine, Customized MiSeq sequencing, Multiplexed PCR-directed BS-amplicons, Multiplexed barcoding of target amplicon libraries, Next-generation sequencing, Targeted bisulfite sequencing
Référence
BMC Genomics. 2017 01 18;18(1):96