Comparison of oocyte vitrification using a semi-automated or a manual closed system in human siblings: survival and transcriptomic analyses.
Fiche publication
Date publication
décembre 2022
Journal
Journal of ovarian research
Auteurs
Lien Pubmed
Résumé
Indications of oocyte vitrification increased substantially over the last decades for clinical and ethical reasons. A semi-automated vitrification system was recently developed making each act of vitrification reproducible. In this study, we evaluated the efficiency of the semi-automated technique of oocyte vitrification by survival rate, morphometric assessment and resistance to empty micro-injection gesture as compared with a manual method. Additionally, we intended to evaluate transcriptomic consequences of both techniques using single-cell RNA-seq technology.
Mots clés
Assisted reproductive technology, Cryopreservation, Oocyte, Single-cell RNA-seq, Vitrification
Référence
J Ovarian Res. 2022 12 5;15(1):128