The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions.
Fiche publication
Date publication
décembre 2022
Journal
Cell reports
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SERAPHIN Bertrand
Tous les auteurs :
Mauxion F, Basquin J, Ozgur S, Rame M, Albrecht J, Schäfer I, Séraphin B, Conti E
Lien Pubmed
Résumé
The evolutionary conserved CCR4-NOT complex functions in the cytoplasm as the main mRNA deadenylase in both constitutive mRNA turnover and regulated mRNA decay pathways. The versatility of this complex is underpinned by its modular multi-subunit organization, with distinct structural modules actuating different functions. The structure and function of all modules are known, except for that of the N-terminal module. Using different structural approaches, we obtained high-resolution data revealing the architecture of the human N-terminal module composed of CNOT1, CNOT10, and CNOT11. The structure shows how two helical domains of CNOT1 sandwich CNOT10 and CNOT11, leaving the most conserved domain of CNOT11 protruding into solvent as an antenna. We discovered that GGNBP2, a protein identified as a tumor suppressor and spermatogenic factor, is a conserved interacting partner of the CNOT11 antenna domain. Structural and biochemical analyses thus pinpoint the N-terminal CNOT1-CNOT10-CNOT11 module as a conserved protein-protein interaction platform.
Mots clés
AlphaFold, CCR4-NOT complex, CP: Molecular biology, GGNBP2, X-ray crystallography, cryo-EM, deadenylation, mRNA decay, protein-protein interactions
Référence
Cell Rep. 2022 12 23;:111902