Recent Advances and Current Trends in Transmission Tomographic Diffraction Microscopy.
Fiche publication
Date publication
février 2024
Journal
Sensors (Basel, Switzerland)
Auteurs
Membres identifiés du Cancéropôle Est :
Pr HAEBERLE Olivier
Tous les auteurs :
Verrier N, Debailleul M, Haeberlé O
Lien Pubmed
Résumé
Optical microscopy techniques are among the most used methods in biomedical sample characterization. In their more advanced realization, optical microscopes demonstrate resolution down to the nanometric scale. These methods rely on the use of fluorescent sample labeling in order to break the diffraction limit. However, fluorescent molecules' phototoxicity or photobleaching is not always compatible with the investigated samples. To overcome this limitation, quantitative phase imaging techniques have been proposed. Among these, holographic imaging has demonstrated its ability to image living microscopic samples without staining. However, for a 3D assessment of samples, tomographic acquisitions are needed. Tomographic Diffraction Microscopy (TDM) combines holographic acquisitions with tomographic reconstructions. Relying on a 3D synthetic aperture process, TDM allows for 3D quantitative measurements of the complex refractive index of the investigated sample. Since its initial proposition by Emil Wolf in 1969, the concept of TDM has found a lot of applications and has become one of the hot topics in biomedical imaging. This review focuses on recent achievements in TDM development. Current trends and perspectives of the technique are also discussed.
Mots clés
data reconstruction, diffraction, fourier optics, holographic microscopy, holography, multiple scattering, polarimetric/vectorial imaging, tomography
Référence
Sensors (Basel). 2024 02 29;24(5):