In vitro effects of wound-dressings on key wound healing properties of dermal fibroblasts.
Fiche publication
Date publication
mai 2024
Journal
Experimental dermatology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BENSUSSAN Armand
Tous les auteurs :
Peltier S, Adib Y, Nicosia L, Ly Ka So S, Da Silva C, Serror K, Duciel L, Proust R, Mimoun M, Bagot M, Bensussan A, des Courtils C, Michel L
Lien Pubmed
Résumé
Healing of complex wounds requires dressings that must, at least, not hinder and should ideally promote the activity of key healing cells, in particular fibroblasts. This in vitro study assessed the effects of three wound-dressings (a pure Ca alginate: Algostéril®, a Ca alginate + carboxymethylcellulose: Biatain alginate® and a polyacrylate impregnated with lipido-colloid matrix: UrgoClean®) on dermal fibroblast activity. The results showed the pure calcium alginate to be non-cytotoxic, whereas the other wound-dressings showed moderate to strong cytotoxicity. The two alginates stimulated fibroblast migration and proliferation, whereas the polyacrylate altered migration and had no effect on proliferation. The pure Ca alginate significantly increased the TGF-β-induced fibroblast activation, which is essential to healing. This activation was confirmed by a significant increase in Vascular endothelial growth factor (VEGF) secretion and a higher collagen production. The other dressings reduced these fibroblast activities. The pure Ca alginate was also able to counteract the inhibitory effect of NK cell supernatants on fibroblast migration. These in vitro results demonstrate that tested wound-dressings are not equivalent for fibroblast activation. Only Algostéril was found to promote all the fibroblast activities tested, which could contribute to its healing efficacy demonstrated in the clinic.
Mots clés
dermal fibroblasts, healing, wound‐dressing
Référence
Exp Dermatol. 2024 05;33(5):e15098