Probing of Nucleic Acid Structures, Dynamics, and Interactions With Environment-Sensitive Fluorescent Labels.
Fiche publication
Date publication
février 2020
Journal
Frontiers in chemistry
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DZIUBA Dmytro
Tous les auteurs :
Michel BY, Dziuba D, Benhida R, Demchenko AP, Burger A
Lien Pubmed
Résumé
Fluorescence labeling and probing are fundamental techniques for nucleic acid analysis and quantification. However, new fluorescent probes and approaches are urgently needed in order to accurately determine structural and conformational dynamics of DNA and RNA at the level of single nucleobases/base pairs, and to probe the interactions between nucleic acids with proteins. This review describes the means by which to achieve these goals using nucleobase replacement or modification with advanced fluorescent dyes that respond by the changing of their fluorescence parameters to their local environment (altered polarity, hydration, flipping dynamics, and formation/breaking of hydrogen bonds).
Mots clés
emissive nucleobase, fluorescence sensing, nucleoside analog, probing interactions, probing nucleic acids
Référence
Front Chem. 2020 02 28;8:112