Thickness determination of hydroperoxidized lipid bilayers from medium-resolution cryo-TEM images.
Fiche publication
Date publication
avril 2024
Journal
Methods in enzymology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SCHMUTZ Marc
Tous les auteurs :
Lafarge E, Marques CM, Schmutz M, Muller P, Schroder AP
Lien Pubmed
Résumé
As the primary products of lipid oxidation, lipid hydroperoxides constitute an important class of lipids generated by aerobic metabolism. However, despite several years of effort, the structure of the hydroperoxidized bilayer has not yet been observed under electron microscopy. Here we use a 200 kV Cryo-TEM to image small unilamellar vesicles (SUVs) made (i) of pure POPC or SOPC, (ii) of their pure hydroperoxidized form, and (iii) of their equimolar mixtures. We show that the challenges posed by the determination of the thickness of the hydroperoxidized bilayers under these observation conditions can be addressed by an image analysis method that we developed and describe here.
Mots clés
Cryo-TEM, Hydroperoxidation, Lipid bilayers, Small unilamellar vesicles
Référence
Methods Enzymol. 2024 04 23;700:329-348