knock-down decreases tumoral character of colorectal cancer cells and .
Fiche publication
Date publication
janvier 2022
Journal
American journal of cancer research
Auteurs
Membres identifiés du Cancéropôle Est :
Dr DESCHUYTER Marlène
Tous les auteurs :
Deschuyter M, Leger DY, Verboom A, Chaunavel A, Maftah A, Petit JM
Lien Pubmed
Résumé
Tumor cells have a modified glycosylation profile that promotes their evolution and/or their maintenance in the tumor. Sialylation is a type of glycosylation that is often altered in cancers. RNA-Seq database analysis revealed that the sialyltransferase gene is significantly overexpressed at all stages of colorectal cancer (CRC). ST3GAL2 sialylates both glycoproteins and glycolipids. The aim of this work was to investigate the involvement of ST3GAL2 in CRC. Using the HT29 tumor cell line derived from a stage II of CRC, we decreased the expression of by specific shRNA, and then characterized these cells by performing functional tests. We found that knock down (KD) significantly decreases tumor cell proliferation, cell migration and invasiveness properties . The cell cycle of these cells is affected with a change in cell cycle distribution and an increase of cell apoptosis. The effect of KD was then studied , following xenografts into nude mice, in which the tumor progression was significantly reduced. This work demonstrates that ST3GAL2 is a major player in the behavior of colorectal tumor cells, by modifying the sialylation state of glycoproteins and glycolipids which remain to be specifically identified.
Mots clés
HT29, ST3GAL2, biopsies, colorectal cancer, xenografts
Référence
Am J Cancer Res. 2022 01 15;12(1):280-302