Single-cell mapping of leukocyte immunoglobulin-like receptors in kidney transplant rejection.
Fiche publication
Date publication
août 2022
Journal
Frontiers in transplantation
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BONNOTTE Bernard
Tous les auteurs :
Lamarthée B, Genet C, Cattin F, Danger R, Giral M, Brouard S, Van Loon E, Callemeyn J, Naesens M, Anglicheau D, Bonnotte B, Legendre M, Rebibou JM, Tinel C
Lien Pubmed
Résumé
Leukocyte immunoglobulin-like receptors (LILRs) are a family of inhibitory or stimulatory receptors expressed by immune cell types belonging to both myeloid and lymphoid lineage. Several members of the LILR family recognize major histocompatibility complex class I and thus play important roles in a range of clinical situations including pregnancy. Moreover, paired immunoglobulin-like receptors (PIRs), the murine orthologs of LILRs, are implicated in experimental transplant allorecognition by monocytes and contribute to the induction of donor-specific monocyte-memory. After non-self recognition, activating PIRs are transiently overexpressed at the surface of monocytes and participate in donor-specific monocyte recruitment, leading to graft rejection . In the present study, we mapped LILR expression and also their respective reported ligands at single cell level in the renal allograft and circulating cells in the context of kidney transplant rejection. Recipient-derived monocytes were shown to infiltrate the donor tissue and to differentiate into macrophages. We thus also investigate LILR expression during monocyte-to-macrophage differentiation in order to characterize the myeloid population that directly contribute to allorecognition. Altogether our results emphasize non-classical monocytes and CD68+ M1 macrophages as key players in LILRs-ligand interaction in kidney transplantation.
Mots clés
LILR, allorecognition, kidney transplantation, monocytes, single-cell RNA sequencing
Référence
Front Transplant. 2022 08 11;1:952785