A toolbox for enzymatic modification of nucleic acids with photosensitizers for photodynamic therapy.
Fiche publication
Date publication
août 2024
Journal
RSC chemical biology
Auteurs
Membres identifiés du Cancéropôle Est :
Dr FIGLIOLA Carlotta
Tous les auteurs :
Niogret G, Chériaux C, Bonhomme F, Levi-Acobas F, Figliola C, Ulrich G, Gasser G, Hollenstein M
Lien Pubmed
Résumé
Photodynamic therapy (PDT) is an approved cancer treatment modality. Despite its high efficiency, PDT is limited in terms of specificity and by the poor solubility of the rather lipophilic photosensitizers (PSs). In order to alleviate these limitations, PSs can be conjugated to oligonucleotides. However, most conjugation methods often involve complex organic synthesis and result in the appendage of single modifications at the 3'/5' termini of oligonucleotides. Here, we have investigated the possibility of bioconjugating a range of known PSs by polymerase-mediated synthesis. We have prepared a range of modified nucleoside triphosphates by different conjugation methods and investigated the substrate tolerance of these nucleotides for template-dependent and -independent DNA polymerases. This method represents a mild and versatile approach for the conjugation of single or multiple PSs onto oligonucleotides and can be useful to further improve the efficiency of the PDT treatment.
Référence
RSC Chem Biol. 2024 08 28;5(9):841-852