Intravenous infusion of donor apoptotic leukocytes before transplantation delays allogeneic islet graft rejection through regulatory T cells.
Fiche publication
Date publication
décembre 2012
Auteurs
Membres identifiés du Cancéropôle Est :
Dr PERRUCHE Sylvain, Pr KLEINCLAUSS François
Tous les auteurs :
Mougel F, Bonnefoy F, Kury-Paulin S, Borot S, Perruche S, Kantelip B, Penfornis A, Saas P, Kleinclauss F
Lien Pubmed
Résumé
AIM: This study describes the ability of intravenous donor apoptotic leukocyte infusion before islet transplantation to delay allogeneic graft rejection and implicates regulatory T cells (T(reg)) in the effect. METHODS: Allogeneic FVB (Friend virus B-type) islet transplants were placed under the kidney capsule of BALB/c recipient mice rendered diabetic by streptozotocin. Apoptotic donor leukocytes were infused intravenously 7 days before transplantation. Foxp3/DTR/GFP transgenic C57BL/6 mice were used as recipients to show depletion of T(reg) after apoptotic cell infusion. Control mice received islet transplants without apoptotic cells. RESULTS: The graft median survival time (MST) in recipient mice was 15+/-1.5 days when apoptotic cells were infused 7 days prior to transplantation of a 1000-islet-containing allograft and 6+/-0.5 days in the control mice (P
Référence
Diabetes Metab. 2012 Dec;38(6):531-7