A strategy for direct mapping and identification of mutations by whole-genome sequencing.
Fiche publication
Date publication
septembre 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Dr JARRIAULT Sophie
Tous les auteurs :
Zuryn S, Le Gras S, Jamet K, Jarriault S
Lien Pubmed
Résumé
Mutant screens have proven powerful for genetic dissection of a myriad of biological processes, but subsequent identification and isolation of the causative mutations are usually complex and time consuming. We have made the process easier by establishing a novel strategy that employs whole-genome sequencing to simultaneously map and identify mutations without the need for any prior genetic mapping.
Référence
Genetics. 2010 Sep;186(1):427-30