Identification of gene expression profiles correlated to tumor progression in a preclinical model of colon carcinogenesis
Fiche publication
Date publication
juin 2010
Auteurs
Membres identifiés du Cancéropôle Est :
Pr MARESCAUX Jacques, Pr SOLER Luc
Tous les auteurs :
Bousserouel S, Kauntz H, Gosse F, Bouhadjar M, Soler L, Marescaux J, Raul F
Lien Pubmed
Résumé
The rat azoxymethane (AOM)-induced colon carcinogenesis model provides useful information for understanding human colorectal neoplasia. Here, we used the AOM model to measure the gene expression profiles of biomarkers related to tumor progression. We assessed tumor progression stages by computed tomographic (CT) colonography. Messenger RNAs were isolated from tumors and mucosal samples, and gene expression levels were assessed by real-time quantitative polymerase chain reaction (PCR). We show that early stages of tumor progression are associated with an upregulation of matrix metalloproteinase-7 (MMP-7) and of genes involved in the inflammatory response, including interleukin (IL1 beta) and tumor necrosis factor-alpha (TNF alpha). The ratio of B-cell lymphoma/leukemia 2 (Bcl-2)-associated X proteins (Bax) to Bcl-2 transcript (proapototic/antiapoptotic signals) is elevated in early stages of tumor progression (Bax/Bcl-2 >1) and reversed in more advanced stages of tumor development (Bax/Bcl-2
Référence
Int J Oncol. 2010 Jun;36(6):1485-90.